Topsil® Series HPLC Column
Topsil® series HPLC column is a next-generation column by Welch, besides Ultisil® , Xtimate® and Welchrom®. The series use different silica and provide different selectivity.
Features
- High purity silica (99.99%) with 150 Å pore size and 260 m²/g surface area
- 12% carbon loading for C18 phase
- Because of large pore and moderate carbon loading, Topsil C18 phase can also be used as AQ-C18 without phase collapse
- Endcapped for excellent peak shape and lifetime
- Lower back pressure than Ultisil, almost the same column efficiency as Ultisil
- Good for small molecules and peptides
- Topsil phase including C18, C8, Phenyl-Hexyl, Silica, NH2 and CN
Topsil® C18
| Structural Formula |  |
| pH Range | 2.0-9.5 |
| Particle Size | 3 µm, 5 µm |
| Surface Area(m²/g) | 260(150 Å) |
| Carbon Loading(%) | 12(150 Å) |
| USP List | L1 |
| Endcapped | Yes |
Topsil® C8
| Structural Formula |  |
| pH Range | 2.0-9.5 |
| Particle Size | 3 µm, 5 µm |
| Surface Area(m²/g) | 260(150 Å) |
| Carbon Loading(%) | 10(150 Å) |
| USP List | L7 |
| Endcapped | Yes |
Topsil® NH2
| Structural Formula |  |
| pH Range | 2.0-8.0 |
| Particle Size | 5 µm |
| Surface Area(m²/g) | 260(150 Å) |
| Carbon Loading(%) | 3(150 Å) |
| USP List | L8 |
| Endcapped | No |
Topsil® CN
| Structural Formula |  |
| pH Range | 2.0-8.0 |
| Particle Size | 5 µm |
| Surface Area(m²/g) | 260(150 Å) |
| Carbon Loading(%) | 6(150 Å) |
| USP List | L10 |
| Endcapped | Yes |
Topsil® Silica
| Structural Formula |  |
| pH Range | 2.0-8.0 |
| Particle Size | 5 µm |
| Surface Area(m²/g) | 260(150 Å) |
| Carbon Loading(%) | N/A |
| USP List | L3 |
| Endcapped | No |
Topsil® Phenyl-Hexyl
| Structural Formula |  |
| pH Range | 2.0-9.5 |
| Particle Size | 3 µm, 5 µm |
| Surface Area(m²/g) | 260(150 Å) |
| Carbon Loading(%) | 12(150 Å) |
| USP List | L11 |
| Endcapped | Yes |
Topsil® HILIC NH2
| Structural Formula |  |
| pH Range | 2.0-8.0 |
| Particle Size | 5 µm |
| Surface Area(m²/g) | 260(150 Å) |
| Carbon Loading(%) | 3(150 Å) |
| USP List | L8 |
| Endcapped | No |
Compound Salvia Tablets
| Column: | Topsil® C18, 250×4.6 mm, 5 µm | ||
| Mobile Phase: | A: acetonitrile | B: Water | |
Gradient Program: |
Time(min) | A(%) | B(%) |
| 0 | 19 | 81 | |
| 35 | 19 | 81 | |
| 55 | 71 | 29 | |
| 70 | 71 | 29 | |
| 100 | 40 | 60 | |
| Flow Rate: | 1.0 mL/min | ||
| Temperature | 30°C | ||
| Detector: | 203 nm | ||
| Injection Volume: | 20 µm | ||
Epigeal Srephaia Root
| Column: | Topsil® C18, 250×4.6 mm, 5 µm |
| Mobile Phase: | 25 mM sodium acetate buffer(2% trimethylamine, adjust pH to 3.50 with acetic acid) |
| Flow Rate: | 1.5 mL/min |
| Temperature: | 40°C |
| Detector: | 280 nm |
| Injection Volume: | 20 µm |
Ketoprofen
| Column: | Topsil® C18, 150×4.6 mm, 5 µm |
| Mobile Phase: | Phosphate buffer (68g KH2PO4 dissolve in 1000 mL water, adjust pH to 3.5 with acetic acid) |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 30°C |
| Detector: | 233 nm |
| Injection Volume: | 20 µm |
Vitamin D3
| Column: | Topsil® Silica, 250×4.6 mm, 5 µm |
| Mobile Phase: | N-hexane/n-amyl alcohol=99.7/0.3 |
| Flow Rate: | 2.0 mL/min |
| Temperature: | 30°C |
| Detector: | 254 nm |
| Injection Volume: | 20 µL |
Sex hormone in Cosmetics
| Column: | Topsil® Phenyl-Hexyl, 250×4.6 mm, 5 µm |
| Mobile Phase: | Methanol/Water = 60/40 |
| Flow Rate: | 1.0 mL/min |
| Temperature: | 30°C |
| Detector: | 210 nm, 254 nm |
| Injection Volume: | 20 µL |
| Mixed Standards: | Estrogen: estradiol, oestrone, estrostilben, estriol Androgen: testosterone, Methyltestosterone Progestational hormone:progesterone |
