UHPLC COLUMN

Welch also offers Ultisil® UHPLC (1.8μm) columns. With high column efficiency and good lot-to-lot reproducibility, Ultisil® UHPLC can generate high quality data, decreasing the probability of repeated sample analyses while reducing the consumption of solvent at the same time. Ultisil® UHPLC series offer a variety of bonded phases, specified guard columns and pre-columns for the users to design and realize faster and more environmentally friendly chromatography applications with higher resolution.

 

Feature

  • Ultra resolution: same resolution as or better than that of conventional column which has more packing materials.
  • Ultra speed: UHPLC offers more information per unit time and higher speed owing to its smaller particles.
  • Sensitivity: higher N, narrower peak width (W), higher peak height. The system sensitivity of 1.8μm UHPLC is 70% and 40% higher than that of conventional colμmn of 5μm and 3.5μm packings, respectively.

 


 

 Ultisil® UHPLC XB-C18

Structural Formula  
pH Range 1.5-10.0
Particle Size 1.8μm
Surface Area(m2/g) 320(120Å)
Carbon Loading(%) 17(120Å)
USP List L1
Endcapped Yes

 

Ultisil® UHPLC AQ-C18

Structural Formula  
pH Range 1.5-10.0
Particle Size 1.8μm
Surface Area(m2/g) 320(120Å)
Carbon Loading(%) 12(120Å)
USP List L1
Endcapped Yes

 


 

 

Column Packing Features

  • Unique column packing technique
  • Withstand ultra-high pressure of
    UHPLC instruments

Hardware Features

New design
Low dead volume
New special frit

Packing Materials Features

  • High efficiency 1.8μm particles
  • High column efficiency and excellent strength
  • Variety of bonding chemistries
  • Stable column bed, highest pressure: 15000psi

 

 

Analysis of Aflatoxin

Column: Ultisil® UHPLC XB-C18, 2.1×100mm, 1.8μm
Mobile Phase: Methanol/acetonitirile/water=18/18/64
Flow Rate: 0.35mL/min
Detector: FLD Excitation:365 nm Emission:450 nm
Temperature: 35°C
Injection Volume: 2μL
Instrument: Waters UPLC
Samples in Order: G2, G1, B2, B1

 

 

Analysis of Telmisartan Tablets

Sample Name RT Area USP Theoretical
Plate Number
Telmisartan 3.222 487938 126585
Telmisartan 3.222 487646 126607
Telmisartan 3.222 488317 126791

 

Inline Filter for UHPLC

  P/N Description
00808-01221 UltraShield inline Fliter, SS, 0.5μm stainless steel frit, 15000psi
00808-01222 Direct Connect Precolumn inline Filter, with 0.2μm
Replacement Frits×5, 18000psi
00808-UF020 Replaceable frits (0.2μm)

 

 

Ordering Information—1.8μm UHPLC column

Particle
Size
ID
(mm)
Column Length (mm) Guard
Cartridge
Cartridge
Holder
30 50 75 100 150 10mm length  
Ultisil
XB-C18
2.1 H00201-11009 H00201-11010 H00201-11011 H00201-11012 H00201-11014 HU808-201-25 00808-01109
3.0 H00201-11018 H00201-11019 H00201-11020 H00201-11021 H00201-11023 HU808-201-25 00808-01109
4.6 H00201-11036 H00201-11037 H00201-11038 H00201-11039 H00201-11041 HU808-201-45 00808-01109
Ultisil
AQ-C18
2.1 H00207-11009 H00207-11010 H00207-11011 H00207-11012 H00207-11014 HU808-207-25 00808-01109
3.0 H00207-11018 H00207-11019 H00207-11020 H00207-11021 H00207-11023 HU808-207-25 00808-01109
4.6 H00207-11036 H00207-11037 H00207-11038 H00207-11039 H00207-11041 HU808-207-45 00808-01109

 

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