Ultisil® Ion Exchange Column

Ultisil® ion exchange columns are available for both Strong Anion Exchange(SAX) and Strong Cation Exchange(SCX) columns. The SCX/SAX columns are silica based with high resolutio ad high efficiecy.

Ultisil® SAX is a polar bonded phase, consisting of an ammonium-functionalized silane while Ultisil® SCX is a classical strog cation.

 

Features

  • Organic modifiers such as acetoitrile and methanol maybe used with SAX and SCX columns, within organic/buffer solubility constraints
  • Retention can be controlled by varying pH, ionic strength and organic modifier content.

 


 

 

Ultisil® XB-SAX

Structural Formula  
pH Range 2.0-8.0
Particle Size 3μm, 5μm, 10μm
Surface Area(m2/g) 320(120Å), 90(300Å)
Carbon Loading(%) 7.5(120Å), 1.5(300Å)
USP List L14
Endcapped No

 

 

Ultisil® XB-SCX

Structural Formula  
pH Range 2.0-8.0
Particle Size 3μm, 5μm, 10μm
Surface Area(m2/g) 320(120Å), 90(300Å)
Carbon Loading(%) 12(120Å), 5(300Å)
USP List L9
Endcapped No

 

 

13 Heparin Disaccharides

Column: Ultisil® XB-SAX, 3.0×250mm, 5μm
Mobile Phase: A: weight 0.308g NaH2PO4 to 1000mL volumetric
flask, add 950mL water to dissolve it, adjust pH
2.9 with H3PO4, then add water to scale mark
B: weight 122g NaClO4 to 1000mL volumetric
flask, add 950mL mobile phase A to dissolve,
adjust pH 3.0 with H3PO4, then add mobile phase
A to scale mark.
Flow Rate: 0.45mL/min
Detector: 234nm, 202nm
Temperature: 50°C
Injection Volume: 10μL

 

 

Chondroitin Sulfate

Column:  Ultisil® XB-SAX, 4.6×250mm, 5μm
Mobile Phase:  A: water, adjust pH 3.5 with diluted HCl
B: 2mol/L NaCl, adjust pH 3.5 with diluted HCl
Flow Rate:  1.0mL/min
Detector:  232nm
Temperature:  Ambient
Injection Volume:  20μL
Mixed Standards: Chondroitin disaccharide(B)
6- sulfated chondroitin disaccharide(C)
4- sulfated chondroitin disaccharide(A)

 

 

Orazamide

Column:  Ultisil® XB-SCX, 4.6×250mm, 5μm
Mobile Phase:  1.0% NH4H2PO4( pH 3.0 adjusted by H3PO4)
Flow Rate:  1.0mL/min
Detector:  215nm
Temperature:  30°C
Injection Volume:  10μL
Samples In Order: Orotic acid, Pyridinepropanimidamide,
4-Amino-5-imidazolecarboxamide
hydrochloride and AZO

 

 

Metformin HCL

Column:  Ultisil® XB-SCX, 4.6×250mm, 5μm
Mobile Phase:  1.7% NH4H2PO4( pH 3.0 adjusted by H3PO4)
Flow Rate:  1.0mL/min
Detector:  218nm
Temperature: Ambient
Injection Volume:  10μL
Samples In Order: Icyandiamide, melamine, metformin HCL
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