Ultisil® XB-C18 ----- Universal HPLC Analytical Column
Ultisil® XB-C18 is the most commonly used column in the market. It can substitute Waters Symmetry C18, Phenomenex Luna C18, Supelcosil LC-18-D8, YMC ODS-AM, Alltima C18, GL, Inertsil ODS-2 etc. XB-C18 has high theoretical plates and peak capacity, so it's suitable for analysis of complex samples.
Ultisil® XB-C18
| Structural Formula |  |
| pH Range | 1.5-10.0 |
| Particle Size | 3μm, 5μm, 10μm |
| Surface Area(m²/g) | 320(120 Å), 30(300 Å) |
| Carbon Loading(%) | 17(120 Å), 8(300 Å) |
| USP List | L1 |
| Endcapped | Yes |
Separation of Basic Compounds Antidepressant at pH 7.0
| Column: | Ultisil® XB-C18, 4.6×150 mm, 5 µm | |
| Mobile Phase: | 20 mM phosphote(pH 7.0) /methanol=20/80 | |
| Flow rate: | 1.0 mL/min | |
| Detector | 215 nm | |
| Temperature: | 25°C | |
| Sample |
1)Uracil 3)Ortriptyline 5)Trimipramine |
2)Ropranolol 4)Amitryptyline |
Comparison of Peak Shape between Batch to Batch
| Column: | Ultisil® XB-C18, 4.6×250 mm, 5 µm | |
| Mobile Phase: | Methanol / water=75/ 25 | |
| Flow rate: | 1.0 mL/min | |
| Detector: | 254 nm | |
| Temperature: | 25°C | |
| Samples: |
1)Uracil 3)4-chloronitrobenzen |
2)Phenol 4)Methylbenzene |
Capacity Factor(K’) of Batch to Batch Reproducibility
Excellent Stability at Low pH and High pH
The stability of XB-C18 is better than other brand columns under pH 1.3 or under pH 10.
Argratoban
| Column: | Ultisil® XB-C18, 4.6×250 mm, 3 µm | ||
| Mobile Phase: | A: 10mmol/L ammonium acetate B: acetonitrile/methanol=50/30 |
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| Gradient Program: |
Time(min) 0 20 35 50 60 60.1 80 |
A(%) 60 60 50 20 20 60 60 |
B(%) 40 40 50 80 80 40 40 |
| Flow Rate: | 0.6mL/min | ||
| Detector: | 259nm | ||
| Temperature: | 50°C | ||
| Injection Volume: | 10μL | ||
| Reference Sample: | S-argatroban, R-argatroban | ||
Paracetamol Injection USP 36
| Column: | Ultisil® XB-C18, 4.6×250 mm, 5 µm | ||
| Mobile Phase: | A: methanol/water/acetic acid =50/950/1 B: methanol/water/acetic acid =50/500/1 |
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| Gradient Program: |
Time(min) 0 8 53 58 59 73 |
A(%) 82 82 0 0 82 82 |
B(%) 18 18 100 100 18 18 |
| Flow Rate: | 0.9mL/min | ||
| Detector: | 254nm, 275nm, 317nm | ||
| Temperature: | 40°C | ||
| Injection Volume: | 20μL | ||
| Reference Sample: | L-hydroxyproline, glycine, alanine, L-proline | ||
Aflatoxin
| Column: | Ultisil® XB-C18, 4.6×250mm, 5μm |
| Mobile Phase: | Water/methanol/acetonitrile=46/40/14 |
| Flow Rate: | 1.0mL/min |
| Detector: |
Excitation wavelength: 360nm |
| Temperature: | 30°C |
| Injection Volume: | Post -column photo chemical derivation (254 nm) |
| Aflatoxin B1, B2, G1, G2 mixed standards, meets separation requirements |
|
Progesterone(EP 5.0)
| Column: | Ultisil® XB-C18, 4.6×150mm, 5μm | ||
| Mobile Phase: | A: water B: acetonitrile | ||
| Gradient Program: |
Time(min) 0-20 20-27 27-45 45-50 |
A(%) 50 50-20 20 50 |
B(%) 50 50-80 80 50 |
| Flow Rate: | 0.9mL/min | ||
| Detector: | 254nm, 275nm, 317nm | ||
| Temperature: | 40°C | ||
| Injection Volume: | 20μL | ||
| Reference Sample: | L-hydroxyproline, glycine, alanine, L-proline | ||
