Ultisil® XB-C8 -------------Less retentive than XB-C18
The XB-C8 phase is less retentive than XB-C18 phase, useful for strong hydrophobic compounds that are too strongly retained on C18 phase, and for LC/MS applications, where long retention is not desire. When seperating neutral or other highly retained compounds, XB-C8 can save analytical time. However, when seperating polar compounds, XB-C8 column provides differentselectivity than does XB-C18 column.
Ultisil® XB-C8
| Structural Formula |  |
| pH Range | 1.5-10.0 |
| Particle Size | 3μm, 5μm, 10μm |
| Surface Area(m²/g) | 320(120 Å), 90(300 Å) |
| Carbon Loading(%) | 12(120 Å), 4(300 Å) |
| USP List | L7 |
| Endcapped | Yes |
Comparison of Retention of XB-C18 and XB-C8
| Column: | 4.6×150mm, 5μm | |
| Mobile Phase: | Water/acetonitrile=30/70 | |
| Flow rate: | 1.0mL/min | |
| Detector: | 344nm | |
| Temperature: | 25°C | |
| Samples: |
1) Uracil 2) Ethylbenzene 3) Propylbenzene |
4) Butylbenzene 5) Amylbezene 6) Heptylbenzene |
| Column: | 4.6×150mm, 5μm | |
| Mobile Phase: | 20mM phosphate(pH 7.0)/methanol=20/80 | |
| Flow rate: | 1.0mL/min | |
| Detector: | 215nm | |
| Temperature: | 25°C | |
| Samples: |
1) Uracil 2) Ropranolol 3) Ortriptyline |
4) Amitryptyline 5) Trimipramine |
Tirucallol and Euphol
| Column: | Ultisil® XB-C8, 4.6×250mm, 5μm |
| Mobile Phase: | Acetonitrile/water=90/10 |
| Flow rate: | 1.0mL/min |
| Detector: | 210nm |
| Temperature: | 30°C |
| Samples: | 10μL |
Nalbuphine HCl
| Column: | Ultisil® XB-C8, 4.6×250mm, 5μm | ||
| Mobile Phase: | A: 0.02mol/L KH2PO4 buffer(pH 6.5)(%) B: acetonitrile |
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| Gradient Program: |
Time(min) 0 15 25 28 30 45 |
A(%) 80 70 40 40 80 80 |
B(%) 20 30 60 60 20 20 |
| Flow rate: | 1.0mL/min | ||
| Detector: | 220nm | ||
| Temperature: | 30°C | ||
| Injection Volume: | 10μL | ||
Analysis of Insulin Detemir
| Column: | Ultisil® XB-C18, 4.6×250mm, 5μm |
| Mobile Phase: | A: 20g (NH4)2SO4, 900mL water, 100mL acetonitrile, adjust pH 2.3 B: acetonitrile/water=80/20; %B=0(0min) , 30(9min), 60(40min) |
| Flow rate: | 1.0mL/min |
| Detector: | 214nm |
| Temperature: | 30°C |
| Injection Volume: | 20μL |
