Ultisil® XB-NH2
Ultisil® XB-NH2 column is based on propyl-amino silane, mostly used in normal phase, but can also be used in reversed phase
Features
- Used in normal phase for weak anion-exchange, and in reversed-phase HPLC for polar compound
- For applications in aggressive normal phase mode with aqueous eluent
- Vitamins A and D can be separated in the normal-phase mode
- Carbohydrates and sugars can be seperated in the reversed-phase mode
Utisil® XB-NH2
| Structural Formula |  |
| pH Range | 2.0-8.0 |
| Particle Size | 3μm, 5μm, 10μm |
| Surface Area(m²/g) | 320(120 Å) |
| Carbon Loading(%) | 4(120 Å) |
| USP List | L8 |
| Endcapped | No |
Acarbose
| Column: | Ultisil® XB-NH2, 4.6×250 mm, 5 µm |
| Mobile Phase: | Phosphate buffer */acetonitrile = 28/72 * Dissolve 600 mg of s KH2PO4 and 279 mg of ADSP in 100 mL water, add water to make 1000 mL |
| Detector: | 210 nm |
| Temperature: | 35°C |
| Flow Rate: | 2.0 mL/min |
| Injection Volumne: | 10 µL |
Acetyl/-L -carnitine
| Column: | Ultisil® XB-NH2, 4.6×250 mm, 5 µm |
| Mobile Phase: | Buffer/acetonitrile = 30/70 |
| Detector: | 205 nm, 210 nm |
| Temperature: | 20°C |
| Flow Rate: | 1.0 mL/min |
| Injection Volumne: | 10 µm |
Seperation of N-tert-butylglycine hydrochloride and N-tert-butylglycine acid chloride hydrochloride
| Column: | Ultisil® XB-NH2, 4.6×250 mm, 5 µm |
| Mobile Phase: | Methanol/isopropanol = 80/20 |
| Detector: | 210 nm |
| Temperature: | 30°C |
| Flow Rate: | 1.0 mL/min |
| Injection Volumne: | 5 µL |
Ornithine Aspartate
| Column: | Ultisil® XB-NH2, 4.6×250 mm, 5 µm |
| Mobile Phase: | KH2PO4 buffer solution*/acetonitrile = 40/60 * Dissolve 2.72 g of KH2PO4 in 500 mL water, add 5 mL of concentrated ammonia solution, add water to 1000 mL, adjust pH 5.60±0.05 with H3PO4 |
| Detector: | 205 nm |
| Temperature: | 30°C |
| Flow Rate: | 1.0 mL/min |
| Injection Volumne: | 20 µL |
