Welchrom® Series HPLC Column

—Combination of perfect peak shape and lowest back pressure

Feature

  • Perfect peak shape and low back pressure.
  • Ultra-high purity (>99.999%) Type B silica particles.
  • New Bonding and endcapping technique.
  • Economically priced.

 

 Welchrom® C18

Structural Formula  
pH Range 1.5-10.0
Particle Size 5μm
Surface Area(m2/g) 320(120Å)
Carbon Loading(%) 19(120Å)
USP List L1
Endcapped Yes

 

 

Welchrom® C8

Structural Formula  
pH Range 1.5-10.0
Particle Size 5μm
Surface Area(m2/g) 320(120Å)
Carbon Loading(%) 12(120Å)
USP List L7
Endcapped Yes

 

 

Comparison with other brands

Brands Tailing factor(amitriptyline) Back pressure(Methanol/H2O)=75/25
Welchrom® XB-C18 1.29 77 bar
Chrom C18 1.52 108 bar
Sino Chrom C18 1.71 106 bar
BinChrom C18 1.67 102 bar

 

 

Butenafine HCL

Column: Welchrom® C18, 4.6×250mm, 5μm
Mobile Phase: Acetate buffer/methanol/isopropanol = 17/70/13
Flow Rate: 1.0mL/min
Detector: 282 nm
Temperature: Ambient
Injection Volume: 10μL

 

Pentoxyverine citrate

Column: Welchrom® C18, 4.6×150mm, 5μm
Mobile Phase: Water* /methanol=45/55
* Dilute 10mL triethylamine to 1000mL, adjust
pH 3.0 with H3PO4
Flow Rate: 1.0mL/min
Detector: 215 nm
Temperature: 30°C
Injection Volume: 20μL

 

Tanshinone IIA in Salvia Miltiorrhiza

Column: Welchrom® C18, 4.6×250mm, 5μm
Mobile Phase: Methanol/water=75/25
Flow Rate: 1.0mL/min
Detector: 270 nm
Temperature: 22°C

 

Imidacloprid

Column: Welchrom® C18, 250×4.6mm, 5μm
Mobile Phase: Methanol/water=45/55
Flow Rate: 1.0mL/min
Detector: 260 nm
Temperature: 25°C

 

 

Malic acid

Column: Welchrom® C8, 4.6×250mm, 5μm
Mobile Phase: Phosphoric acid/methanol/water=1/100/900
Flow Rate: 0.8mL/min
Detector: 214 nm
Temperature: 20°C
Injection Volume: 5μL
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