Xtimate® HPLC Column
Besides C18 and C8 bonded phases, Xtimate also provides C4, CN, Phenyl bonded phases.
Xtimate® applies a new special SmoothpakTM technique to C18, C8, C4, CN, Phenyl and amino phases, different than the bonding method of other series. As a result, Xtimate® provides a different selectivity, improved stability and reproducibility. In particular, for the Phenyl phase of Phenyl-Hexyl, Xtimate® is totally different from Ultisil® Phenyl. Xtimate® Phenyl-Hexyl phase’s longer hexyl group provides extra hydrocarbon interaction and longer retention than conventional phenyl-propyl phase; it also provides better chemical stability.
Welch also adds polar embedded phase, Polar-RP on Xtimate® particles, to further improve peak shape for very polar and
strong basic compounds and provides different selectivity than does C18 phase.
Xtimate® C18
| Structural Formula |  |
| pH Range | 1.0-12.5 |
| Particle Size | 3μm, 5μm, 10μm |
| Surface Area(m2/g) | 320(120Å) |
| Carbon Loading(%) | 14(120Å) |
| USP List | L1 |
| Endcapped | Yes |
Xtimate® C8
| Structural Formula |  |
| pH Range | 1.0-12.5 |
| Particle Size | 3μm, 5μm, 10μm |
| Surface Area(m2/g) | 320(120Å),100(300Å) |
| Carbon Loading(%) | 10(120Å), 5(300Å) |
| USP List | L7 |
| Endcapped | Yes |
Rosuvastatin Calcium
| Column: | Xtimate® C18, 3.0×150mm, 3μm | ||
| Mobile Phase: | A: 1% TFA/acetonitrile/water=1/29/70 B: 1% TFA/acetonitrile/water=1/75/24 |
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| Gradient Program: |
Time(min) 0-30 30-50 50-60 60-70 |
A(%) 100 100-50 60-0 0 |
B(%) 0 0-40 40-100 100 |
| Flow Rate: | 0.75mL/min | ||
| Detector: | 242nm | ||
| Temperature: | 40°C | ||
| Injection Volume: | 10μL | ||
Cangrelor
| Column: | Xtimate® C18, 4.6×250mm, 5μm | ||
| Mobile Phase: | A: 0.05 mol/L K2HPO4(pH 8.5) B: acetonitrile |
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| Gradient Program: |
Time(min) 0 3 35 50 60 65 66 75 |
A(%) 95 95 67 60 35 35 95 95 |
B(%) 5 5 33 40 65 65 5 5 |
| Flow Rate: | 1.0mL/min | ||
| Detector: | 242nm | ||
| Temperature: | 25°C | ||
| Injection Volume: | 5μL | ||
Omeprazole
| Column: | Xtimate® C8, 250×4.6mm, 5μm | ||
| Mobile Phase: | 10mmoL/L Na2HPO4(pH7.4)/Acetonitrile=70/30 | ||
| Flow Rate: | 1.0mL/min | ||
| Detector: | 280nm | ||
| Temperature: | Ambient | ||
| Injection Volume: | 20μL | ||
Enalapril Maleate
| Column: | Xtimate® C8, 250×4.6mm, 5μm | ||
| Mobile Phase: | Phostphate buffer/acetonitrile = 75/25 | ||
| Flow Rate: | 1.0mL/min | ||
| Detector: | 280nm | ||
| Temperature: | 50°C | ||
| Injection Volume: | 20μL | ||
